PacBio Sequel

 The PacBio Sequel is the newest sequencing platform released by Pacific Biosciences. The PacBio Sequel, aptly named, follows the release of the PacBio RS II System; one of the first to offer read lengths greater than 20Kb, and the PacBio Sequel is no different. In fact, while the Sequel has a much smaller footprint than its predecessor, the Sequel System is able to provide 7x the amount of reads as the RS II.  Where the RS II was able to generate up to 1 Gb of data, the Sequel is able to generate 3-8Gb of data. The driving force behind this long read sequencing technology is what PacBio calls SMRT, or Single Molecule, Real Time technology. This SMRT technology combined with Zero-Mode Waveguides (ZMWs) allow the PacBio Sequel to offer some of the longest read lengths the scientific community has ever seen. Read lengths ranging from 10Kb all the way to 60kb make the PacBio Sequel the ideal system for whole genome sequencing and de novo sequencing. With flexible run times and an increased amount of data generation, the PacBio Sequel provides long read sequencing at an affordable cost.

 

 

    Low Coverage (< 500 Seqs): $50/assay
Medium Coverage (~5,000 Seqs): $150/assay
PacBio Sequel Long Read Amplicon Sequencing High Coverage (~10,000): $250/assay
(16s, 18s, ITS, and Custom) Custom Coverage available; Pricing based on linear increase of 10K seqs pricing
    For projects < 10 samples a $100 library fee is added & $20/sample PCR replication fee

PacBio SEQUEL:

 

Long read amplicon sequencing for 16s, 18s, ITS and custom amplicons e.g. near full 16s ~1400bp, ITS1-4, 18s ~1700bp or other custom amplicon diversity (700-2500bp)

 

Usually a minimum of 10 samples or a small project fee applies and a $20/sample additional PCR replication fee.

 

 

Pricing

$150/sample  for 5,000 sequences per assay,

 

$250 for 10K

 

$500 for $20K

 

**Custom assays from 700bp - 3000bp or larger of course have a barcoding fee that is typically $15/barcode

While the long read sequencing technology of the Sequel does cater to those interested in whole genome and de novo sequencing, the PacBio Sequel has proven to be a valuable tool in 16s and metagenomic studies. Previously, scientists have had to pick and choose which region of the 16s gene they would like to sequence due to read length limitations, but with the Sequel added to our sequencing portfolio, researchers now have the ability to sequence the complete 16s rRNA gene. The Sequel is one of the first sequencing platforms able to provide long sequencing reads at a high-throughput level, and as such will be one of the most sought after platforms to complete taxonomic and phylogenetic studies.

PacBio SMRT Applications

MRDNA Bacteria Sequencing Contact Number

    Low Coverage (< 500 Seqs): $50/assay
Medium Coverage (~5,000 Seqs): $150/assay
PacBio Sequel Long Read Amplicon Sequencing High Coverage (~10,000): $250/assay
(16s, 18s, ITS, and Custom) Custom Coverage available; Pricing based on linear increase of 10K seqs pricing
    For projects < 10 samples a $100 library fee is added & $20/sample PCR replication fee